Journal: bioRxiv

Article Title: Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to vaccination

doi: 10.1101/2023.05.23.541927

Figure Lengend Snippet: Transcriptional features of ST3-reactive and non-ST3-reactive B cells elicited by vaccination. A) Representative staining of ST3-reactive B cells from unvaccinated and vaccinated samples, gated on IgG+ B cells (Live CD3 - CD19 + CD20 + IgG + ). B) Frequency of ST3-reactive IgG+ B cells in vaccinated and unvaccinated monkeys. C, D) Correlation between ST3-reactive IgG+ B cells and ST3-specific IgG (C) and OPA (D) titers. E, F) UMAP of single-cell transcriptomes colored by sort fraction (E) and cell phenotype (F). Dot plot showing scaled expression and percent of cells expressing B cell marker genes in each transcriptional phenotype. H) Volcano plot of differentially expressed transcripts between B2M hi and MBC-like cells. P-values are calculated using a two-sided Wilcoxon rank-sum test and are adjusted using Bonferroni correction.

Article Snippet: Cells were then washed three times in FBS staining buffer (BD) and were then stained with BV605-conjugated anti-IgM (MHM-88), BV786-conjugated anti-CD20 (both from Biolegend), DyLight 405-congjuatated anti-CD19 (CB19) (R&D systems), V500-conjugated CD3 (SP34-2), PE-Cy5-conjugated anti-IgG (G18-145) (both from BD), and Total-seq A anti-human hashtag antibody (Biolegend) on ice for 30 minutes.

Techniques: Staining, Expressing, Marker

Journal: STAR Protocols

Article Title: Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics

doi: 10.1016/j.xpro.2022.101402

Figure Lengend Snippet: Flow cytometry enriched antibody cocktail (22 μL) panel for analyzing rarer granulocyte and myeloid cells in mouse spleen for downstream scRNA-seq

Article Snippet: CD19 Antibody, anti-mouse, PE, REAfinityTM Clone REA749 , Miltenyi Biotec , Cat# 130-112-035; RRID: AB_2655822.

Techniques: Flow Cytometry, Marker

Journal: STAR Protocols

Article Title: Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics

doi: 10.1016/j.xpro.2022.101402

Figure Lengend Snippet:

Article Snippet: CD19 Antibody, anti-mouse, PE, REAfinityTM Clone REA749 , Miltenyi Biotec , Cat# 130-112-035; RRID: AB_2655822.

Techniques: Recombinant, Sterility, Saline, Extraction, Blocking Assay, Software, Solvent, Sequencing, RNA Sequencing, Transferring, Aerosol, Irradiation, Inverted Microscopy, Flow Cytometry, Fluorescence